INTRODUCTION:

An Ayurvedic formulation called Shata Dhauta Ghrita, where "Shata" refers to one hundred, "Dhauta" refers to washing and “Ghrita” refers to ghee. Through this procedure, ghee is turned into a nutritious, cooling, and silky cream that is frequently used as an anti-wrinkle and moisturizer. Ghee is prepared by washing it in water until it warms up, then discarding it and starting over with new water a procedure that can be repeated up to a hundred times¹.

Shata Dhauta Ghrita turns into a smooth, uniform, and non-greasy product in comparison with ordinary ghee, which has an overpowering smell and a granular, oily texture. This enhances patient compliance by making it simpler to apply. Additionally, Shata Dhauta Ghrita's neutral pH provides protection from skin irritation, while ghee's pH is acidic. Shata Dhauta Ghrita reduced particle size results in it being non-granular, non-sticky, and uniform, and making application easier and possibly increasing skin absorption. A smooth oil-in-water emulsion with enhanced consistency and viscosity is produced by the washing process, which makes it perfect for topical application².

The process described above turns ghee into a nourishing, cooling, silky, and smooth cream that can be used as an anti-wrinkle cream and moisturizer. Cow ghee's characteristic smell and granular, oily texture are eliminated by washing it 100 times, resulting a uniform, smooth, and non-oily formulation that is simpler to use and enhances patient compliance. Shata Dhauta Ghrita is a better alternative to traditional ghee because of its neutral pH, which prevents skin irritation. Its reduced particle size ensures that the product is uniform, non-granular, and non-sticky, which enhances application and possibly increases skin absorption. Additionally, the washing procedure produces a smoother, less viscous oil-in-water emulsion that is better suited for topical application³

Natural materials used in the production of herbal cosmetics are becoming more and more popular due to their lower adverse effects when compared to synthetic alternatives. By managing problems like wrinkles, acne, and oil control, these treatments are designed to protect the skin while enhancing its appearance. Many procedures have been developed with both natural and synthetic ingredients, including like sunscreen, anti-aging, and anti-acne creams. Widely used in skincare products, herbal creams use the healing properties of plant-based extracts to protect, nourish, and refresh the skin while providing a mild and efficient alternative to natural skincare products^4,5.

MATERIALS AND METHODS:

Materials: A2 cow ghee, Moringa oil, Mulberry oil, Aloe vera gel, Stearic acid, Borax, Fragrance Tijuca G 105 (Formula 21 labs), Rice Starch and Water.

Table no. 1: Raw Material with Their Uses

Sr. No.	Raw Material	Uses
1.	A2 cow ghee	Moisturizing, Nourishing
2.	Moringa oil	Antioxidant, Antiaging
3.	Mulberry oil	Anti-acne
4.	Aloe vera gel	Soothing effect, Reduce Hyperpigmentation
5.	Steric Acid	Emulsifying Agent, Skin Softener
6.	Borax	pH Adjuster, Preservative
7.	Rice Starch	Absorbs Excess Oil, Enhances Spreadability
8.	Tijuca G 105	Fragrance

Preparation of Base Using A2 Cow Ghee³_.

Perform these procedures to make Shata Dhauta Ghrita the Niragni method (without heat):

Components:

Make by using fresh ghee.

Make sure the water you use for washing is gentle and pure.

Step 1: Mix water and ghee: Combine the ghee and water in a clean container in equal parts (1:1 ratio)

Step 2: Mix the ingredients: In a copper vessel, continuously stir the ghee and water, and wash the ghee 100 times with consistent stirring.

Step 3: Allow the separation: Allow the ghee to naturally separate from the water by allowing the mixture settle for a few minutes.

Step 4: Drain the water: Hold the ghee in the container and carefully drain off the water.

Step 5: Carry out the procedure again: To smooth out and purify the ghee, repeat the washing and separation procedure 10 times in total.

Step 6: Completion of Shata Dhauta Ghrita: Following 10 cycles, the ghee is completely cleansed and prepared for use in Ayurvedic medicines as Shata dhauta Ghrita.

This technique ensures the ghee's purity and medicinal qualities by repeatedly cleaning it without using heat.

Phytoconstituent Analysis:

Phytoconstituent analysis revealed that Moringa oil contains flavonoids, tannins, and vitamins C and E, offering strong anti-aging and antioxidant benefits. Mulberry oil contains tannins, alkaloids, and vitamins C and E, which provide effective anti-acne properties. Aloe vera gel has lignins, amino acids, flavonoids, and anthraquinones, which soothe the skin and help reduce hyperpigmentation.

Preparation of Oil Phase:⁵

Add the Shata Dhauta Ghrita prepared base, Mulberry oil, Aloe vera gel, Stearic acid, Borax, and Moringa oil in the right amounts to a clean porcelain dish. Heat the mixture to 75°C, stirring constantly, until the Stearic acid and other solids melt into a smooth, uniform oil phase. Once fully melted, remove from heat. This oil phase will later be combined with the water phase in the cream-making process.

Preparation of Aqueous Phase:⁵

Prepare a clean porcelain dish with the right amount of water and Rice Starch. Heat the mixture to 75°C, stirring constantly, until the Rice Starch dissolves completely and the mixture becomes smooth and well-blended. Once ready, this aqueous phase can be mixed with the oil phase in the next steps of the cream-making process.

Preparation of Cream:⁵

At 75°C, the aqueous phase was slowly mixed with the oil phase while being constantly stirred. After the transfer was finished, the liquid was continuously stirred while it cooled to room temperature. Just before putting the finished product in an appropriate container, Perfume was incorporated. After that, the herbal multipurpose cream's overall appearance was evaluated.

Formulation Table:

Table No. 2: Formulation Table with Their Prescribed Quantity

Sr. No.	Ingredients	F1	F2	F3
1.	Ghee	4g	10g	20g
2.	Moringa oil	1.5g	3.75g	7.50g
3.	Mulberry oil	1.5g	3.75g	7.50g
4.	Aloe vera gel	4g	10g	20g
5.	Steric acid	1g	2.5g	5g
6.	Borax	0.2g	0.5g	1g
7.	Rice starch	0.8g	2g	4g
8.	Water	1.5ml	15ml	30ml
9.	Fragrance	QS	QS	QS

EVALUATION TEST

1. Physical Evaluation:^6–8

Colour: The consistency and acceptability of its colour.

Odour: A pleasing scent without any offensive odour’s.

Texture: The feel of it on the skin, whether it's rough or silky.

Consistency: The proper thickness or fluidity for the purpose for which it is designed.

2. pH:^6,7,9

To calibrate the pH meter, a standard buffer solution was used. After weighing and dissolving around 0.5 g of the cream in 50.0 ml of distilled water, the pH of the mixture was determined.

3. Viscosity:^6,7,9

A Brookfield viscometer (Labman LMDV-60) adjusted to 60 rpm and spindle number 4 can be used to measure the viscosity of formulations.

4. Irritation Test:^6,7,9

The left-hand dorsal surface was marked with a 1 cm² region. After applying the cream to this location, the time was noted. Edema, erythema, and irritation were noted and recorded at regular intervals for up to 24 hours.

5. Spreadability Test:^6,7,9

After placing sufficient cream between two glass slides, a 100-gram weight has been adjusted on the top slide for five minutes. By tracking how the higher slide moves over the lower slide or how long it takes to separate the slides, the spreadability can be determined. The formula

S = m x l / t

Where,

m is the weight on the upper slide,

l is the distance the upper slide has gone,

t is the time required.

6. Homogeneity Test:⁹

The homogeneity of the formulations was evaluated by physical and visual inspection.

7. After Feel Test:⁶

After applying a certain amount of cream, the quality of emolliency, slipperiness, and residue were evaluated.

8. Washability Test:^6,7,9

To determine how easily the herbal creams can be removed, the treated area should be washed through tap water.

9. Greasiness:^6,7

A thin layer of the cream was applied on the skin, and its greasy or oily texture was examined.

10. Microbial Growth Test:⁹

After preparing agar media, the formulated cream was inoculated onto the agar plates using the streak plate method, and a control was made by making the cream eliminated. The plates were then incubated for 24 hours at 37°C, and when the incubation was over, the plates were taken out, and the microbial growth was noted and examined with the control.

RESULT AND DISCUSSION:

1. Preliminary Evaluation:

The formulated cream's colour, odour, texture, consistency, state, and appearance were evaluated in order to determine its physical attributes.

Table No. 3: Preliminary Evaluation

Test	F1	F2	F3
Colour	White	White	White
Odour	Pleasant	Pleasant	Pleasant
Texture	Smooth	Smooth	Smooth
Consistency	Semi Solid	Semi Solid	Semi Solid

3. Physical Evaluation:

The cream's pH ranged from 5.6 to 6.9, which is appropriate for human skin; 6.8 is the optimum pH. Its viscosity (689.4 to 689.8 cps) indicates that it is easily spreadable. After 24hours of use, the cream did not produce any skin irritation, edema, or inflammation. It left no residue behind and showed good homogeneity, emollience, spreading properties and non-greasy. With its thin texture and easy application, the formulation was easily removed using tap water.

Table No. 5: Physical Evaluation

Test	F1	F2	F3
pH	6.7	6.8	6.9
Viscosity	689.4	689.6	689.8
Irritation	No irritation	No irritation	No irritation
Spreadability	Good	Good	Good
Homogeneity	Good	Good	Good
After Feel	Good	Good	Good
Washability	Easily Washed	Easily Washed	Easily Washed
Greasiness	Non-greasy	Non-greasy	Non-greasy

4. Microbial Evaluation:

After a 24-hour incubation period at 37ºC, no signs of growth of bacteria were seen, and the outcomes were comparable to the control.

Table No. 6: Microbial Growth Test

Test	F1	F2	F3
Microbial Growth

CONCLUSION:

Shata Dhauta Ghrita was used to make an herbal multipurpose cream that indicated excellent physical and phytoconstituent benefits. Its viscosity of 689.4 to 689.8 cps ensures a simple application, and its pH range of 6.7 to 6.9 is good for skin compatibility. After a full day of use, there were no side effects, irritation, or edema. The cream was very smooth, simple to spread, washable, non-greasy left no residue. The existence of beneficial substances such as flavonoids, tannins, and vitamins C and E was discovered through phytoconstituent testing. Additionally, there was no microbiological development in the cream, indicating that it was well preserved. In conclusion, patient compliance is increased by the cream's safety, effectiveness, and superior skin care.

REFERENCES:

1. Parihar DBK, Kadibagil DVR, Lakshkar A. Pharmaceutical Study of Shatadhauta Ghrita Prepared by Two Different Methods.

2. Deshmukh MD, Patil MP, Ahire ED, Gosavi SB. Shatdhauta Ghrita: A Promising agent in the development of herbal creams. J Pharm Negat Results. 2022; 13(1).

3. Shinde PS, Kedar TR. Formulation of Shata Dhauta Ghrita: As A Base in Moisturizing Cream. 2024; 9(3).

4. Sharma A, Prasar B. Formulation and Evaluation of herbal cosmetic cream to produce multipurpose effect on skin. 2013;

5. Modi J, Rathore S, Dwivedi S, Saraogi G. Formulation and Evaluation of Multipurpose Herbal Cream. Int J Newgen Res Pharm Healthc. 2024 Jun 30; 129–34.

6. Bhavani MS, Naveena Ch, Nagamani P, Sowmya B. Formulation and Evaluation of Herbal Face Cream. Int J Pharm Sci Rev Res [Internet]. 2023 Dec [cited 2025 Mar 29]; 83(2). Available from: http://globalresearchonline.net/ijpsrr/v83-2/11.pdf

7. D. Dhangar P, Shimpi H, Newadkar R, Bhadane V, Desale L, Jaiswal N. Formulation and Evaluation of Herbal Extract of Butterfly Pea Multipurpose Cream. Res J Top Cosmet Sci. 2023 Nov 28; 85–90.

8. Bhoge AS, Gangurde AB, Khade PP, Salve DMT. Formulation And Evalution of Moisturizing Cream Using a Shata Dhauta Ghrita as a Base. 2024; 10(3).

9. Bhoge AS, Gangurde AB, Khade PP, Salve DMT. Formulation and Evalution of Moisturizing Cream Using a Shata Dhauta Ghrita as a Base. 2024; 10(3).

Received on 03.05.2025 Revised on 12.10.2025

Accepted on 09.01.2026 Published on 18.04.2026

Available online from April 25, 2026

Research J. Topical and Cosmetic Sci. 2026; 17(1):1-4.

DOI: 10.52711/2321-5844.2026.00001

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. Creative Commons License.

Name	Phytoconstituent	Test	Result
Moringa oil	Flavonoids	Alkaline Reagent Test	Positive
	Tannins	Ferric Chloride Test	Positive
	Vitamin C	Ferric Chloride Test	Positive
	Vitamin E	Iodine Test	Positive
Mulberry oil	Tannins	Ferric Chloride Test	Positive
	Alkaloids	Dragendorff’s Test	Positive
	Vitamin C	Ferric Chloride Test	Positive
	Vitamin E	Iodine Test	Positive
Aloe vera gel	Lignins	Phenolic Test	Positive
	Amino Acids	Ninhydrin Test	Positive
	Flavonoids	Alkaline Reagent Test	Positive
	Anthraquinones	Borntrager’s Test	Positive